This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practising cell culture will benefit greatly from this book.